Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

High throughput detection of telomerase expression by gold nanoprobes assay (nano- TRAP assay): An alternative method to conventional method of telomerase detection

(2015) High throughput detection of telomerase expression by gold nanoprobes assay (nano- TRAP assay): An alternative method to conventional method of telomerase detection. Molecular Medicine Journal.

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Abstract

Introduction: Telomeric Repeat Amplification Protocol assay (TRAP assay) is a standard established technique to detect the activity (expression) of telomerase in the cancerous samples. It includes a time-consuming procedure that has significant drawbacks such as high cost, complicacy, time-consuming procedure, equipment-dependent steps and false-positive signals caused by inclusion of PCR inside the assay. Methods: In this paper we introduce an assay that takes the advantages of gold nanoparticles in order to avoid application of PCR and post-PCR techniques while remaining highly sensitive, specific and quantitative enough. In addition, our proposed method is much simpler, cheaper and reliable than standard TRAP assay. We call this new technique “nano-TRAP assay” as it recruits gold nanoparticles for signal amplification step. Results: In nano-TRAP assay, telomerase elongates a suitable primer, and then gold nanoparticle-probe conjugates (gold nanoprobes) could be used to recognize the elongated products of telomerase. After that, gold or silver enhancement step could be done to translate the presence of a few gold nanoprobe-elongated primer complex to a measurable signal (signal enhancement). This enhanced signal could be inspected by naked eye, optical density measurement and analysis of their scanned images by image analysis tools. Conclusion: Nano-TRAP assay is an easy to use, low-cost and rapid alternative to conventional TRAP assay and since it is not very equipment demanding, it could be done in low-resources laboratories.

Item Type: Article
Keywords: Nano-TRAP assay, Gold nanoprobe, Conventional TRAP assay, Telomerase
Subjects: genetic
Divisions: Research Vice-Chancellor Department > Molecular Medicine Journal
Depositing User: Molecular Medicine Journal
URI: http://eprints.hums.ac.ir/id/eprint/961

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