Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

An “olivomycin A” derivative from a sponge-associated Streptomyces sp. strain SP 85

(2019) An “olivomycin A” derivative from a sponge-associated Streptomyces sp. strain SP 85. 3 Biotech.

[img] Text
olivomycin A.pdf

Download (1MB)

Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

We isolated an actinobacterium, Streptomyces sp. strain SP 85 from the marine sponge Dysidea avara. Polyphasic identification of the microorganism showed that the strain SP 85 had high 16S rRNA gene similarity (99) with Streptomyces olivaceus strain NBRC 12805, while some physiological and biochemical differences were observed. A cytotoxic compound, SP 85 was isolated from the active culture extract of the strain SP 85 by bioassay-guided purification over silica gel column chromatography, preparative TLC, and HPLC. The structure elucidation based on the spectroscopic analysis, including UV, ESI–MS, and 13C NMR data revealed that SP 85 compound is an analog of anti-tumor drug, “olivomycin A”. The SP 85 compound showed high cytotoxic activity against three human cancer cell lines, including SW480, HepG2, and MCF7 with IC50 values of 16, 93, and 78 nM, respectively. SP 85 exhibited significantly (2–10 times) higher cytotoxicity against the tumor cell lines in comparison with HUVECs as the normal cell line, which also induced apoptosis in the tested cancerous cell line. This is the first report on the production of an “olivomycin A” derivative by a sponge-associated Streptomyces, showing the great potential of sponge-associated actinobacteria in producing cytotoxic natural products. © 2019, King Abdulaziz City for Science and Technology.

Item Type: Article
Additional Information: cited By 0
Keywords: antineoplastic antibiotic; doxorubicin; olivomycin derivative; RNA 16S; unclassified drug, apoptosis; Article; bacterial strain; bacterium identification; bacterium isolation; carbon nuclear magnetic resonance; cell death; column chromatography; controlled study; cytotoxicity; DNA fragmentation assay; Dysidea avara; gene sequence; Hep-G2 cell line; human; human cell; HUVEC cell line; IC50; isolation and purification; liquid chromatography; liquid chromatography-mass spectrometry; MCF-7 cell line; MTT assay; nonhuman; priority journal; reversed phase high performance liquid chromatography; sequence homology; sponge (Porifera); Streptomyces; SW480 cell line; thin layer chromatography; ultraviolet spectroscopy
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
Divisions: Research Vice-Chancellor Department > Molecular Medicine Research Center
Depositing User: هدی فهیم پور
URI: http://eprints.hums.ac.ir/id/eprint/6681

Actions (login required)

View Item View Item