Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

The expression of Drosha, DGCR8, Dicer and Ago-2 genes are upregulated in human umbilical vein endothelial cells under hyperglycemic condition

(2018) The expression of Drosha, DGCR8, Dicer and Ago-2 genes are upregulated in human umbilical vein endothelial cells under hyperglycemic condition. ENDOCRINE REGULATIONS.

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[Endocrine Regulations] The expression of Drosha DGCR8 Dicer and Ago-2 genes are upregulated in human umbilical vein endothelial cells under hyperglycemic condition.pdf

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Abstract

Objectives. It has been shown that dysregulation of miRNAs expression contributes to the pathogenesis and progression of the diabetes and diabetes-related complications. Drosha, DGCR8, Dicer, and Ago-2 are involved in the miRNA maturation. The aim of the present study was to investigate the mRNA expression levels of these genes in the human umbilical vein endothelial cells (HUVECs) under hyperglycemic condition. Methods. HUVECs were cultured in normo- (5 mM) and hyperglycemic (25 mM) conditions for 24 h. As osmotic control, cells were treated with D-mannitol (25 mM, for 24 h). The mRNA expression levels of Drosha, DGCR8, Dicer and Ago-2 were evaluated using quantitative real-time PCR. Results. The expression level of Drosha, DGCR8, Dicer, and Ago-2 were increased in hyperglycemic HUVECs compared to the control group. Conclusion. Our results show that under hyperglycemic condition, expression of genes involved in the miRNA maturation was significantly increased in HUVECs. Upregulation of these genes may have role in diabetic complications through the dysregulation of the miRNA expression.

Item Type: Article
Keywords: hyperglycemia, Drosha, DGCR8, Dicer, Ago-2, human umbilical vein endothelial cells
Subjects: endocrine
Divisions: Research Vice-Chancellor Department > Molecular Medicine Journal
Depositing User: هدی فهیم پور
URI: http://eprints.hums.ac.ir/id/eprint/5895

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