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Hormozgan University of Medical Sciences

Comparative prevalence of blaCTX-m-15 gene with virulence genes and serotypes in klebsiella pneumoniae

(2018) Comparative prevalence of blaCTX-m-15 gene with virulence genes and serotypes in klebsiella pneumoniae. Jundishapur Journal of Microbiology.

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Abstract

Background: A newly emerged hypervirulent strain of Klebsiella pneumoniae has caused great concern globally; however, its molecular characteristics have been rarely reported in Iran. Objectives: The goal of this study was to detect the virulence determinants and serotypes of K. pneumoniae and to evaluate the association among selected virulence traits and blaCTX-M-15 gene in southeastern Iran. Methods: One hundred and three non-duplicate K. pneumoniae strains were isolated from clinical samples. The isolates were identified by standard bacteriological tests. Confirmed isolates were examined to detect a selection of virulence genes (wabG, rmpA and iucB) and serotypes (K1, K2, K5 and K20) by PCR. The isolates were studied foe the presence of beta-lactamase (blaCTX-M-15) gene. SPSS software (version 19.0) was used for data analysis. Results: Among the 103 K. pneumoniae isolates, 61 (59.2) isolates were positive for wabG, 4 (3.9) for iucB and 3 (2.9) for rmpA genes. The presence of K20 in 3.9 (4/103) of the isolates represented the most prevalent. Only 3 (2.9) isolates possessed the K1 serotype, while K2 and K5 serotypes were not detected in any isolate. The blaCTX-M-15 gene was detected in 47 (45.6) isolates. blaCTX-M-15-positive isolates showed a higher prevalence of wabG among the studied isolates (P < 0.05). Conclusions: Our data indicate a correlation between presence of virulence gene and blaCTX-M-15 in K. pneumonia isolates. Further research should be undertaken to unravel aspects of both virulence factors and antibiotic resistance which may probably contribute to managing future spread of infectious diseases. © 2018, Jundishapur Journal of Microbiology.

Item Type: Article
Additional Information: cited By 0
Keywords: antibiotic resistance; Article; bacterial gene; bacterial strain; bacterial virulence; bacterium detection; bacterium isolate; blaCTX M 15 gene; comparative study; controlled study; correlational study; data analysis software; genetic association; iucB gene; Klebsiella pneumoniae; microbiological examination; nonhuman; polymerase chain reaction; prevalence; rmpA gene; serotype; wabG gene
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
Depositing User: مهندس هدی فهیم پور
URI: http://eprints.hums.ac.ir/id/eprint/5678

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