Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

Kinetics of α-glucosidase inhibition by different fractions of three species of Labiatae extracts: a new diabetes treatment model

(2017) Kinetics of α-glucosidase inhibition by different fractions of three species of Labiatae extracts: a new diabetes treatment model. PHARMACEUTICAL BIOLOGY.

[img] Text
vv.pdf
Restricted to Registered users only

Download (1MB)

Abstract

Context: Glucosidases are a group of enzymes playing crucial roles in digestion of carbohydrates. Glucosidase inhibitors can reduce carbohydrate digestion rate and have the potential to prevent development of type 2 diabetes. The Labiatae is one of the largest plant families grown globally and many studies that have isolated new pharmaceutical compounds. In folk medicine, some of Labiatae plants such as Zataria multiflora Boiss, Salvia mirzayanii Rech. F. & Esfand, and Otostegia persica Boiss are consumed for the treatment of diabetes. Objectives: This study investigates the inhibitory effects of different fractions of three mentioned species extracts on a-glucosidase. Materials and methods: Ethanol extracts of these plants leaves were fractionated using petroleum ether, chloroform, ethyl acetate, and n-butanol solutions. The duration of this study was 12months. To measure enzyme inhibition, 5 lL of the enzyme, 20 lL of substrate and samples were used and for evaluation mode of inhibition, constant amounts of a-glucosidase were incubated with rising concentrations of substrate (PNPG). Results: The results revealed that the ethyl acetate fraction of Zataria multiflora (IC50 ¼0.35 ± 0.01mg/mL) and petroleum ether fraction of Salvia mirzayanii (IC50 ¼0.4 ± 0.11mg/mL) were the most potent inhibitors of a-glucosidase in comparison with the other samples and acarbose as the standard (IC50 ¼7 ± 0.19 mg/ mL). All of the samples exhibited noncompetitive-uncompetitive inhibition. Discussion and conclusion: It can be inferred from this study that a-glucosidase inhibitory potential of the studied extracts may be a marker of antidiabetic potential of these extracts.

Item Type: Article
Keywords: diabetes-Glucosidases
Subjects: QT Physiology > QT104-172 Human Physiology
QU Biochemistry. Cell Biology and Genetics > QU 100-133 Biochemistry of the Human Body
QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
Divisions: Research Vice-Chancellor Department > Molecular Medicine Research Center
Depositing User: مرکز تحقیقات پزشکی مولکولی
URI: http://eprints.hums.ac.ir/id/eprint/5642

Actions (login required)

View Item View Item