Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

High expression of fas ligand on cord blood dendritic cells: A possible immunoregulatory mechanism after cord blood transplantation

(2011) High expression of fas ligand on cord blood dendritic cells: A possible immunoregulatory mechanism after cord blood transplantation. Transplantation Proceedings.

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Allogeneic cord blood transplantation is associated with less severe graft-versus-host disease (GVHD). Dendritic cells (DCs), as the most potent antigen-presenting cells of the immune system, play a central role in the development of GVHD. Because apoptosis induction is one of the known mechanisms that DCs use to regulate T-cell responses, we studied the immunostimulatory and apoptosis induction capacities of cord blood dendritic cells (CBDCs) and peripheral blood dendritic cells (PBDCs) to evaluate the mechanisms underlying the lower incidence of GVHD after cord blood transplantation. Presence of apoptosis-related markers Fas, Fas ligand (FasL), and CD40 and costimulatory molecules, along with the proportion of myeloid and lymphoid DCs subsets, were also measured on CBDCs and PBDCs. Fresh CBDCs and PBDCs were isolated from cord and peripheral mononuclear cells as lineage-negative cells by using monoclonal antibodies against CD3, CD11b, CD14, CD16, CD19, CD56, CD34, and CD66b. DCs were cocultured with allogeneic T cells, and the effect of CBDCs and PBDCs on T-cell apoptosis and proliferation were determined through flow cytometric analysis and 3H-thymidine incorporation. Our findings showed that CBDCs markedly augment apoptosis of CD3 + T-cells. FasL expression on CBDCs was significantly higher than on PBDCs. However, there was no difference between Fas expression on CBDCs and PBDCs. Moreover, CBDCs were poor stimulators of allogenic T cells in mixed leukocyte reaction compared with adult peripheral blood DCs. They also displayed decreased expression of HLA-DR and CD86 molecules. The ratio of lymphoid DCs (CD11c -, CD123 +) to myeloid DCs (CD11c +, CD123 -) was also significantly higher in CBDCs compared with PBDCs. It seems that less severe GVHD after cord blood transplantation is due not only to a higher degree of immaturity of CBDCs, but also to delivery of apoptotic signals to the host T cells that recognize allo-MHC molecules on CBDCs in the early phase of immune response. © 2011 Published by Elsevier Inc.

Item Type: Article
Additional Information: cited By 4
Keywords: antigen; CD11b antigen; CD123 antigen; CD14 antigen; CD16 antigen; CD19 antigen; CD3 antigen; CD34 antigen; CD56 antigen; CD66b antigen; Fas ligand; glycoprotein p 15095; HLA DR antigen; monoclonal antibody; thymidine; tritium; unclassified drug, allotransplantation; apoptosis; cell isolation; coculture; conference paper; controlled study; cord blood dendritic cell; cord blood stem cell transplantation; dendritic cell; flow cytometry; human; immunoregulation; lymphocyte proliferation; newborn; normal human; peripheral blood dendritic cell; priority journal; protein expression; T lymphocyte, Apoptosis; Cell Proliferation; Cells, Cultured; Coculture Techniques; Cord Blood Stem Cell Transplantation; Dendritic Cells; Fas Ligand Protein; Fetal Blood; Flow Cytometry; Graft vs Host Disease; Humans; Immunophenotyping; Lymphocyte Culture Test, Mixed; T-Lymphocytes
Subjects: WS Pediatrics > WS 200-342 Diseases of Children and Adolescents
WS Pediatrics > WS 405-460 By Age Groups
Depositing User: مهندس هدی فهیم پور

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