Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

Drug-related mutational patterns in hepatitis B virus (HBV) reverse transcriptase proteins from Iranian treatment-naïve chronic HBV patients

(2013) Drug-related mutational patterns in hepatitis B virus (HBV) reverse transcriptase proteins from Iranian treatment-naïve chronic HBV patients. Hepatitis Monthly.

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Abstract

Background: Immunomodulators and Nucleotide analogues have been used globally for the dealing of chronic hepatitis B virus (HBV) infection. However, the development of drug resistance is a major limitation to their long-term effectiveness. Objectives: The aim of this study was to characterize the hepatitis B virus reverse transcriptase (RT) protein variations among Iranian chronic HBV carriers who did not receive any antiviral treatments. Materials and Methods: Hepatitis B virus partial RT genes from 325 chronic in active carrier patients were amplified and directly sequenced. Nucleotide/amino acid substitutions were identified compared to the sequences obtained from the database. Results: All strains belonging to genotype D.365 amino-acid substitutions were found. Mutations related to lamivudine, adefovir, telbivudine, and entecavir occurred in (YMDD) 4 (n = 13), (SVQ) 17.23 (n = 56), (M204I/V + L180M) 2.45 (n = 8) and (M204I) 2.76 (n = 9) of patients, respectively. Conclusions: RT mutants do occur naturally and could be found in HBV carriers who have never received antiviral therapy. However, mutations related to drug resistance in Iranian treatment-naïve chronic HBV patients were found to be higher than other studies published formerly. Chronic HBV patients should be monitored closely prior the commencement of therapy to achieve the best regimen option. © 2013, KOWSAR Corp.

Item Type: Article
Additional Information: cited By 12
Keywords: adefovir; entecavir; hepatitis B surface antibody; hepatitis B surface antigen; lamivudine; RNA directed DNA polymerase; telbivudine, adult; amino acid substitution; antiviral resistance; article; cross-sectional study; DNA sequence; enzyme linked immunosorbent assay; female; gene mutation; genotype; hepatitis B; Hepatitis B virus; human; Iran; major clinical study; male; mutation rate; mutational analysis; nucleotide sequence; phylogeny; polymerase chain reaction
Subjects: WC Communicable Diseases > WC 500-590 Virus Diseases
WI Digestive System > WI 700-770 Liver. Biliary Tract
Depositing User: مهندس هدی فهیم پور
URI: http://eprints.hums.ac.ir/id/eprint/5151

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