Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

Hepatitis B virus surface protein mutations clustered mainly in CTL immune epitopes in chronic carriers: Results of an Iranian nationwide study

(2013) Hepatitis B virus surface protein mutations clustered mainly in CTL immune epitopes in chronic carriers: Results of an Iranian nationwide study. Journal of Viral Hepatitis.

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Mutations within the coding region of hepatitis B surface antigen (HBsAg) have been found naturally in chronic carriers. To characterize the mutations of HBsAg from Iranian chronic carriers who were vaccine and/or medication naive. The surface genes from 360 patients were amplified and directly sequenced. The distribution of amino acid substitutions was classified according to different immune epitopes of the surface protein. All isolates belonged to genotype D. 222 (61.6) of 360 patients contained at least one amino acid substitution. 404 (74.5) of 542 amino acid changes occurred in different immune epitopes of HBsAg, of which 112 (27.7) in 32 residues of B-cell epitopes (62 in the 'a' determinant); 111 (27.4) in 32 residues of T helper; and 197 (48.7) in 32 residues inside cytotoxic T lymphocyte (CTL) epitopes. One Th (186-197) and two CTL (28-51 and 206-215) epitopes were found to be hotspot motifs for the occurrence of 213 (52.7) substitutions. 20 stop codons were identified in different epitopes. There was a significant association between amino acid substitutions and anti-HBe seropositivity; however, the correlation between such changes with viral load and ALT levels was not significant. In chronic hepatitis B virus(HBV) carriers, positive selection in particular outside the 'a' determinant appeared to exert influence on the surface proteins. These changes could be immune escape mutations naturally occurring due to the host immune surveillance especially at the T-cell level. © 2013 John Wiley & Sons Ltd.

Item Type: Article
Additional Information: cited By 13
Keywords: alanine aminotransferase; hepatitis B surface antigen, adult; alanine aminotransferase blood level; amino acid substitution; article; B lymphocyte; cytotoxic T lymphocyte; female; gene sequence; genetic variability; genotype; helper cell; Hepatitis B virus; human; immunosurveillance; major clinical study; male; missense mutation; priority journal; stop codon; virus load, Adult; Amino Acid Substitution; Carrier State; Cross-Sectional Studies; DNA, Viral; Epitopes, T-Lymphocyte; Female; Hepatitis B Surface Antigens; Hepatitis B virus; Hepatitis B, Chronic; Humans; Immune Evasion; Iran; Male; Middle Aged; Mutation, Missense; Sequence Analysis, DNA; T-Lymphocytes, Cytotoxic
Subjects: WC Communicable Diseases > WC 500-590 Virus Diseases
WI Digestive System > WI 700-770 Liver. Biliary Tract
Depositing User: مهندس هدی فهیم پور

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