Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

Upregulation of microRNA-146a was not accompanied by downregulation of pro-inflammatory markers in diabetic kidney

(2013) Upregulation of microRNA-146a was not accompanied by downregulation of pro-inflammatory markers in diabetic kidney. Molecular Biology Reports.

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The present study was designed to evaluate whether microRNA-146a and its adapter proteins (TRAF6 and IRAK1) are involved in the pathogenesis of diabetesinduced kidney damage. Male Sprague-Dawley rats were divided into control and diabetic groups (n = 6 in each). Diabetes was induced by injection of streptozotocin (55 mg/kg; i.p.) in 12 h fasted rats. Diabetic kidney damage was diagnosed by renal hypertrophy, thickened glomerular basement membrane, widened filtration slits, mesangial expansion, as well as by elevated levels of blood urea and creatinine in diabetic rats 2 months after induction of diabetes. While the expression of NF-kB mRNA and miR-146a were increased in diabetic kidney compared to the sham controls (p<0.01 for both comparisons), the mRNA levels of IRAK1 and TRAF6 did not statistically reduce. The NF-jB activity and the concentrations of TNF-α, IL-6 and IL-1b in the kidney of diabetic rats were higher than the kidney of controls (p<0.05 for TNF-α and NF-kB; p<0.01 for IL-6 and IL-1β). Our results indicate that the upregulation of miR-146a was not accompanied by downregulation of inflammatory mediators in diabetic kidney. It is possible that a defect in the miR-146a-mediated negative loop provides a situation for sustained activation of NF-jB and its targets to promote cells toward abnormalities. © 2013 Springer Science+Business Media Dordrecht.

Item Type: Article
Additional Information: cited By 22
Keywords: creatinine; insulin; interleukin 1 receptor associated kinase 1; interleukin 1beta; interleukin 6; microRNA 146a; tumor necrosis factor alpha; tumor necrosis factor receptor associated factor 6, animal experiment; animal model; article; cell proliferation; controlled study; creatinine blood level; diabetic nephropathy; down regulation; endothelium cell; insulin blood level; mesangium cell; nonhuman; nucleotide sequence; protein expression; protein function; rat; upregulation; urea blood level, Animals; Biological Markers; Cytokines; Diabetic Nephropathies; Disease Models, Animal; Gene Expression Regulation; Inflammation Mediators; Kidney; Kidney Glomerulus; Male; MicroRNAs; NF-kappa B; Rats
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
Depositing User: مهندس هدی فهیم پور

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