Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

Spoligotyping and drug resistance patterns of Mycobacterium tuberculosis isolates from five provinces of Iran

(2013) Spoligotyping and drug resistance patterns of Mycobacterium tuberculosis isolates from five provinces of Iran. MicrobiologyOpen.

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Tuberculosis (TB) persists as a public health problem in Iran. Characterization of Mycobacterium tuberculosis isolates circulating in this area will contribute to understand and control the spread of the strains. The aims of this study were to understand the genetic diversity and drug susceptibility of M. tuberculosis isolates circulating in Iran and to analyze the relationship between genotype and drug resistance. A total of 291 M. tuberculosis isolates collected from TB patients were genotyped by spoligotyping. Drug susceptibility testing was performed using proportion method. Spoligotyping resulted in 75 distinct patterns. 86.2 of isolates were grouped in 35 clusters while the remaining isolates were unique. Ural was found to be the most predominant lineage (34.3) followed by Central Asian strain (CAS) (24), T (18.2), Manu2 (7.5) and Latin American-Mediterranean (LAM) (6.1). The five largest clusters were Ural/Spoligotype International Type (SIT)127 (15.8), CAS1/SIT26 (9.2), T1/SIT53 (6.1), T1/SIT284 (5.4), and CAS1/SIT25 (4.4). About 5 of isolates had multidrug resistance (MDR) and 10 had other resistance. MDR was significantly associated with Beijing strains, but not with Ural family. This study highlights dominance of Ural, CAS, and T families in Iran. Biogeographic specificity of CAS and T families to border provinces of Iran including Sistan-Baluchestan and Kermanshah, respectively, suggested that this family strains might be transmitted from these regions to other provinces of the country. Spoligotyping was used to study the diversity of Mycobacterium tuberculosis isolates collected from five different provinces of the country. © 2013 The Authors.

Item Type: Article
Additional Information: cited By 12
Keywords: ethambutol; isoniazid; rifampicin; streptomycin, adolescent; adult; aged; antibiotic resistance; article; bacterial cell; bacterial strain; bacterium isolation; biogeography; child; correlation analysis; drug sensitivity; female; genetic variability; genotype; human; Iran; major clinical study; male; multidrug resistance; Mycobacterium tuberculosis; preschool child; priority journal; school child; spoligotyping, Iran; MDR-TB; Mycobacterium tuberculosis; spoligotyping., Antitubercular Agents; Cluster Analysis; Drug Resistance, Bacterial; Genotype; Humans; Iran; Microbial Sensitivity Tests; Molecular Epidemiology; Molecular Typing; Mycobacterium tuberculosis; Phylogeography; Tuberculosis
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
Depositing User: مهندس هدی فهیم پور

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