Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

Purification and Characterization of L- Asparaginase from Bacillus Sp. Pg- 03

(2014) Purification and Characterization of L- Asparaginase from Bacillus Sp. Pg- 03. Iranian Journal of Public Health.

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Official URL: https://www.questia.com/library/journal/1P3-346744...

Abstract

Background: The aim of the present work was the discovery of a new L-asparaginase that is serologically different from the previously reported ones, but has similar therapeutic ef- fects. Methods: A novel Bacillus sp.PG-03 was grown in modified M9 medium and incubated in a rotary shaking incubator. After 24 h of inoculation, the cells were removed by centrifu- gation. Sonication of the cells was carried out to release the intracellular L-asparaginase and DEAE-Cellulose Ion ex- change chromatography was used for purification of the en- zyme. SDS PAGE analysis was performed and the purity and molecular weight of the enzyme was estimated. The activity of L-asparaginase was evaluated at different pH values and temperature. Results: A novel bacterial strain, Bacillus PG-03 was isolated from the Persian Gulf sediments and screened for the ability of L- asparaginase production. …

Item Type: Article
Keywords: L-Asparaginase; Bacillus Sp.PG-03; Leukemia; Enzyme Activity
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
WC Communicable Diseases > WC 195-425 Infection. Bacterial Infections
Depositing User: مهندس هدی فهیم پور
URI: http://eprints.hums.ac.ir/id/eprint/5070

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