Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

Isolation and Molecular Identification of L-Asparaginase Producing Bacteria from Persian Gulf

(2014) Isolation and Molecular Identification of L-Asparaginase Producing Bacteria from Persian Gulf. Iranian Journal of Public Health.

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Official URL: https://www.questia.com/library/journal/1P3-346742...

Abstract

Background: L-asparaginase is an anti-neoplastic agent used in the chemotherapy of lymphoblastic leukemia. Nowadays, this enzyme is derived from bacterial sources. The objective of this study was isolation and molecular identification of L- asparaginase producing bacteria from marine environment in Persian Gulf. Methods: 53 marine bacteria were isolated from sediment and seawater samples obtained from Persian Gulf in Hor- mozgan, Iran. The isolated strains were screened for L- asparaginase activity. Production of L-asparaginase was car- ried out in medium M9. Based on the enzyme activity among 20 strains producing L-asparaginase, 4 isolates with high L- asparaginase activity were selected and identified base on nucleotide sequence of 16S rRNA gene. Results: 4 bacteria isolated were identified as Pseudomonas strains and four strains of Bacillus sp. andPseudomonas sp.(PG01) showed the highest productivity of 1.6 IU, and Bacillus sp. (PG04) showed least productivity of 0.67 IU. Conclusion: This study revealed that bacteria isolated from Persian Gulf may be potential source of enzyme L- asparaginase and Pseudomonas sp. …

Persian Abstract

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Item Type: Article
Keywords: L-Asparaginase; Marine Bacteria; 16S Rrna; Persian Gulf
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
WC Communicable Diseases > WC 195-425 Infection. Bacterial Infections
Depositing User: مهندس هدی فهیم پور
URI: http://eprints.hums.ac.ir/id/eprint/5069

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