Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

MicroRNA-146a and its adapter proteins are affected by diabetes in rat's heart

(2016) MicroRNA-146a and its adapter proteins are affected by diabetes in rat's heart. Bratislava Medical Journal.

[img]
Preview
Text
MicroRNA-146aanditsadapterproteinsareaffectedby.pdf

Download (321kB) | Preview

Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

OBJECTIVES: This study was conducted to explore whether microRNA-146a and its adapter proteins (TNF-α receptor-associated factor 6 (TRAF6) and interleukin-1 receptor-associated kinase 1 (IRAK1)) are affected by diabetes in the rat heart. METHODS: Twelve male Sprague-Dawley rats were randomized into control and diabetic groups (n = 6). Streptozotocin-nicotinamide experimental model was used to induce type 2 diabetes. The gene expression of MicroRNA-146a, nuclear factor-ΚB (NF-ΚB), IRAK1 and TRAF6, as well as NF-ΚB activity, IRAK1 and TRAF6 protein levels were measured. Moreover, NF-ΚB activity was measured in response to miR-146a mimic transfection (20 nmol) in human umbilical vein endothelial cells (HUVECs) under hyperglycemic condition (25 mM D-glucose for 24 h). RESULTS: The expression of MicroRNA-146a was increased in the heart tissue, 2 months after diabetes induction and in HUVECs. Also, the mRNA and protein levels of NF-ΚB, IRAK1 and TRAF6 were increased in the heart of diabetic rats. Moreover, transfection of miR-146a mimic prevented from a significant increase of NF-ΚB activity in hyperglycemic HUVECs. CONCLUSION: Presumably, a defect in the regulation of IRAK1 and TRAF6 can weaken miR-146a regulatory effect and provides a situation for sustained activation of NF-ΚB and its targets to promote cardiac cells toward abnormalities.

Item Type: Article
Additional Information: cited By 0
Keywords: Diabetes; Heart; IRAK1; MicroRNA-146a; NF-ΚB; TRAF6
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Biochemistry
Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Physiology Medical
Depositing User: مهندس هدی فهیم پور
URI: http://eprints.hums.ac.ir/id/eprint/4119

Actions (login required)

View Item View Item