Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

Alteration in inflammation-related miR-146a expression in NF-KB signaling pathway in diabetic rat hippocampus

(2016) Alteration in inflammation-related miR-146a expression in NF-KB signaling pathway in diabetic rat hippocampus. Advanced Pharmaceutical Bulletin.

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Abstract

Purpose: The purpose of the present study is to evaluate the expression of miR-146a gene, its adaptor genes (TRAF6, NF-KB, and IRAK1), and possible changes in the cellular signaling pathway in diabetic hippocampus tissue. Methods: Male Sprague-Dawley rats are randomly selected and divided into control and diabetic (n=6) groups. Diabetes induced by the single-dose injection of nicotinamide 110 mg/kg, (i.p.), 15 min before streptozotocin (50 mg/kg; i.p.) in 12-h fasted rats. The rats are kept at the laboratory for two months. After anaesthetization, hippocampus of the rats was removed in order to measure the expression of miR-146a, NFK-B, IRAK1, and TRAF6 genes using real-time PCR and activity of NF-KB as well as amount of apoptosis rate using ELISA. Results: The results indicated a reduction in expression of miR-146a and an increase in expression of IRAK1, NF-KB, and TRAF6 genes in the hippocampus of diabetic rats compared to control. Also it reveals an increase in the activity of NF-KB and apoptosis rate in the hippocampus of diabetic rats. Conclusion: Our results report the probability that reduction of miR-146a expression in the negative feedback loop between miR-146a and NF-KB increases NF-kB expression and thus intensifies inflammation and apoptosis in hippocampus. © 2016 The Authors.

Item Type: Article
Additional Information: cited By 5
Keywords: glucose; immunoglobulin enhancer binding protein; insulin; microRNA 146a; nicotinamide, animal experiment; animal model; animal tissue; apoptosis; Article; body weight; controlled study; diabetes mellitus; enzyme linked immunosorbent assay; gene; gene expression; gene function; glucose blood level; hippocampal tissue; hippocampus; inflammation; insulin blood level; intracellular signaling; IRAK1 gene; male; microRNA 146a gene; NF KB gene; nonhuman; rat; real time polymerase chain reaction; streptozotocin-induced diabetes mellitus; TRAF6 gene
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
Divisions: Research Vice-Chancellor Department > Molecular Medicine Research Center
Depositing User: مهندس هدی فهیم پور
URI: http://eprints.hums.ac.ir/id/eprint/4080

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