Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

Anti Leishmania activity of Lucilia sericata and Calliphora vicina maggots in laboratory models

(2016) Anti Leishmania activity of Lucilia sericata and Calliphora vicina maggots in laboratory models. Experimental Parasitology.

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Abstract

Use of sterile fly larvae (maggots) of blow flies for the treatment of many different types of skin and soft tissue wounds is called Maggot debridement therapy (MDT). The larvae of blow flies secrete a broad spectrum of compounds with diverse mechanisms of action in the gut and salivary glands called excretion/secretion (ES) products which showed to have antimicrobial activities against Gram negative and positive bacteria. Cutaneous leishmaniasis (CL) which is the common form of leishmaniasis is difficult to treat. In this study, the effect of ES from 2nd and 3rd stages of L. sericata and C. vicina larvae on in vitro Leishmania major amastigote growth in macrophage was evaluated. The effect of ES on Leishmania growth was estimated by assessing the rate of macrophage infection and the number of amastigotes per infected macrophages. In addition, the anti Leishmania activities of larval and ES of L. sericata and C. vicina on the skin lesion induced by L. major infection was evaluated in susceptible BALB/c mice. The results showed that ES of both flies reduced the number of infected macrophages; 2.6 and 1.5-fold using L. sericata ES and C. vicina ES, respectively, and inhibited amastigotes growth in macrophages; 2.03 and 1.36-fold by L. sericata ES and C. vicina ES, respectively as compared to the control group. The results showed that L. sericata ES was significantly more effective than C. vicina ES to inhibit in vitro L. major amastigotes growth, The size of lesion was significantly smaller in BALB/c mice treated with L. sericata ES than treated with C. vicina ES. The results of in vivo experiments suggested that pre-treatment with ES derived from L. sericata may have some protective effects on the development of L. major lesion. Therefore, it seems that maggot ES might be considered as a possible candidate for the treatment of cutaneous leishmaniasis. © 2016 Elsevier Inc.

Item Type: Article
Additional Information: cited By 1
Keywords: meglumine antimonate, amastigote; animal cell; animal experiment; animal model; anthelmintic activity; arthropod larva; Article; Calliphora vicina; Calliphoridae; controlled study; female; in vitro study; in vivo study; Leishmania major; Lucilia sericata; macrophage; maggot therapy; mouse; nonhuman; parasite development; priority journal; protection; skin defect; skin leishmaniasis; therapy effect; analysis of variance; animal; Bagg albino mouse; cell line; debridement; Diptera; growth, development and aging; larva; Leishmania major; metabolism; parasitology; physiology; procedures; randomization, Analysis of Variance; Animals; Cell Line; Debridement; Diptera; Female; Larva; Leishmania major; Macrophages; Mice; Mice, Inbred BALB C; Random Allocation
Subjects: QW Microbiology and Immunology > QW 501-949 Immunology
Depositing User: مهندس هدی فهیم پور
URI: http://eprints.hums.ac.ir/id/eprint/3934

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