Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

EFFECTIVE METHODS FOR APPROPRIATE DIAGNOSIS OF BRUCELLOSIS IN HUMANS AND ANIMALS (REVIEW ARTICLE)

(2014) EFFECTIVE METHODS FOR APPROPRIATE DIAGNOSIS OF BRUCELLOSIS IN HUMANS AND ANIMALS (REVIEW ARTICLE). Online Journal of Animal and Feed Research ).

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Abstract

Brucellosis is one of the most common diseases among human that identification and control of disease transmission methods can promote public health. Clinical signs alone are not sufficient for brucellosis diagnosis. Hence, a sensitive, specific, rapid and inexpensive method is required. Early and appropriate diagnosis of this disease is effective in improving public health as well as disease control and eradication. Several serological tests for probable diagnosis of Brucella infection were used in evaluation of antibodies against Brucella. Using new methods such as Elisa has higher sensitivity and specificity than standard SAT test and complement fixation which can show both G and M immunoglobulins. It is also suitable for examining certain class of immunoglobulin. Research and studies have shown that ELISA is a complete method for in vitro detection of chronic disease, especially when other tests results are negative. In addition to this method, all unique and specific immunoglobulin in tested serum appear with high speed and accuracy. Another diagnostic method is PCR, which has higher sensitivity and specificity in comparison with serologic methods for diagnosis of human brucellosis. PCR shows similar sensitivity as 16srRNA using L7/L12 gene. It can be used in diagnosis of human brucellosis. Another diagnostic method is identification of different forms of IL-10 gene, which is a cytokine. It inactivates macrophages and infects the susceptible subject with brucellosis. Therefore, identification of different forms of IL-10 gene is considered as effective method for diagnosis of the disease. It’s recommended to use this new and effective method because many of these methods can overcome limitations of traditional methods.

Item Type: Article
Keywords: Brucellosis, Diagnosis, Human, Animal
Subjects: WA Public Health > WA 108-245 Preventive Medicine
Divisions: Research Vice-Chancellor Department > Social Determinants in Health Promotion Research Center
Depositing User: مركز تحقيقات عوامل اجتماعی درارتقا سلامت خليج فارس
URI: http://eprints.hums.ac.ir/id/eprint/3891

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