Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

Inducible clindamycin resistance in clinical isolates of Staphylococcus aureus due to erm genes, Iran

(2014) Inducible clindamycin resistance in clinical isolates of Staphylococcus aureus due to erm genes, Iran. IRAN. J. MICROBIOL.

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Abstract

Background and objectives: Resistance to macrolide can be mediated by erm and msrA genes in Staphylococcus aureus. There are the evidences that show erm genes may be causative agent of inducible or constitutive resistance. The aim of this study was to investigate the incidence of inducible clindamycin resistance and determine the most frequency of erm and msrA genes among S. aureus isolates. Materials and Methods: In this study a total of 124 non duplicated clinical isolates of S. aureus were tested with disk diffusion method. All isolates were tested by PCR for mecA, ermA, ermB, ermC and msrA genes. Results: According to PCR results, 48.4% had mecA gene and 51.6% were mecA negative. By phenotypic D-test method, 32.3% revealed inducible resistance and recorded as D and D+. Sensitive and constitutive phenotypes were found in 54.8% and 12.9% of isolates respectively. Inducible clindamycin resistance was more prevalent in MRSA (29%) than MSSA isolates (2.4%). Among studied erm genes, the most frequency genes were ermA and ermC with 41.1% and 17.7% respectively. Three isolates of them had D phenotype, while the PCR results of erm genes were negative. All isolates were negative for ermB or msrA genes. Conclusion: Since S. aureus isolates with inducible resistance may mutate and change to constitutive resistance, to prevent treatment failure, we suggest that inducible resistance test be performed on erythromycin resistant/clindamycin sensitive isolates.

Item Type: Article
Keywords: D- test, Inducible clindamycin resistance, Staphylococcus aureus
Subjects: microbiology
infectious diseases
Divisions: Research Vice-Chancellor Department > Infectious and Tropical Diseases Research Center
Depositing User: مركز تحقيقات بيماريهاي عفوني
URI: http://eprints.hums.ac.ir/id/eprint/2506

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