Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

MicroRNA-146a expression and its intervention in NF-кB signaling pathway in diabetic rat aorta

(2014) MicroRNA-146a expression and its intervention in NF-кB signaling pathway in diabetic rat aorta. ENDOCRINE REGULATIONS.

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Abstract

O bjective. The present study was designed to evaluate whether microRNA-146a, as an NF-кB regulating factor and its adapter proteins (TRAF6 and IRAK1), are affected by diabetes in rat aorta. Methods. Male Wistar rats were randomly divided into control and diabetic groups (n=6 in each). Diabetes was induced by a single injection of streptozotocin (55 mg/kg; i.p.) in 12 h fasted rats. The gene expression of microRNA-146a, NF-κB, IRAK1, and TRAF6 were determined by real time PCR. Results. The expression of microRNA-146a was down-regulated in diabetic aorta when compared with the control group (p<0.05). The mRNA expression levels of NF-кB, TRAF6 and IRAK1 also increased in the diabetic rat aorta when compared with their control counterparts (p<0.01 for all comparisons). Conclusion. These results suggest that down-regulation of microRNA-146a may lead to derangement in NF-кB negative feedback loop, propelling the aorta toward inflammation.

Item Type: Article
Keywords: diabetes, aorta, microRNA-146a, NF-κB, IRAK1, TRAF6
Subjects: physiology
Divisions: Research Vice-Chancellor Department > Molecular Medicine Research Center
Depositing User: مرکز تحقیقات پزشکی مولکولی
URI: http://eprints.hums.ac.ir/id/eprint/1054

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