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Hormozgan University of Medical Sciences

Detection of CTX-Mβ-lactamase in Escherichia coli using Disk diffusion, combined disk, MIC and Multiplex PCR methods

(2013) Detection of CTX-Mβ-lactamase in Escherichia coli using Disk diffusion, combined disk, MIC and Multiplex PCR methods. Life Science Journal.

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Abstract

Abstract: Introduction: β-lactamase enzymes are CTX-Menzymes that hydrolyze cephalotine and cefaloridine better than penicillin, and also preferentially hydrolyze cefotaxime over ceftazidime. The aim of the study was to investigate the prevalenceof ESLB-producing E. coli, and molecular evaluation of CTX-Mβ-lactamases using Multiplex in E. colistrains. Materials and methods: A total of 200 E.coliisolates werecollected from clinical specimens and the antibiotic susceptibility of strains was determined using Disk Diffusion method. The production of ESBL enzymes was determined using Combined Disk,Cefotaxime and Ceftazidime with and without Clavulanic Acid. Also, MIC of Ceftazidime and Cefotaxime was determined with and without Clavulanic Acid using Agar Dilution method. Finally, the presence of CTX-Mgenes was detected by specific primers using Multiplex PCR method. Findings: The drug resistance of separated isolates was determined in relation to 14 anti- Antibiotics. In the Combined Disk method,increase in inhibition zone diameter by ≥ 5mm against ceftazidime and cefotaxime with and without Clavulanic Acid as ESBL producers were identified. There were 155 isolates (77.5%) in Combined Disk method as ESBL-producingE. coli.Amongthese ESLB-producing isolates, 66 ESBL samples contained MICCAZ= 16-32 (μg/ml), and 24 samples contained 64 to 512. Also, 48 samples contained MICCTX=4-8 and 97 samples contained 16 to 512 for CTX. 4/57% of these isolates in Multiplex PCR method were CTX-M-producers. Conclusion: The results show that the production of β-lactamase ESBL enzymes in the strains is high (77.5%), which is confirmed by Agar Dilution and Combined Disk methods. PCR method shows higher prevalence of CTXMenzyme. Therefore, appropriate treatment is recommended in the country to prevent the spread of ESBLproducing Escherichia coli strains.

Item Type: Article
Keywords: E. coli, combined disk, CTX-M, ESBL, MIC, Multiplex PCR
Subjects: molecular Genetics
Divisions: Research Vice-Chancellor Department > Molecular Medicine Research Center
Depositing User: مرکز تحقیقات پزشکی مولکولی
URI: http://eprints.hums.ac.ir/id/eprint/1044

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