Repository of Research and Investigative Information

Repository of Research and Investigative Information

Hormozgan University of Medical Sciences

Detection of Amp-C type Producing Escherichia coliusing the Clavulanic acid and Boronic Acid Inhibitor and Multiplex PCR method

(2013) Detection of Amp-C type Producing Escherichia coliusing the Clavulanic acid and Boronic Acid Inhibitor and Multiplex PCR method. Life Science Journal.

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Abstract

Abstract: Introduction: Amp-C β-lactamases which belong to Cephalosporins family, in comparison with benzyl penicillin, exhibit stronger activity against Cephalosporins like Cefoxitin, Cefotetan and oximino cephalosporin and hydrolyze them. This study was aimed to determine the prevalence of Amp-C producing E.coli and molecular evaluation of 6 sub-groups of respective enzymes among E.coli strains using Multiplex PCR method. Materials and Methods: A total of 200 clinical E.coli isolates were collected from clinical specimens. Antibiotic susceptibility was evaluated by disk diffusion method. Amp-C enzyme production was determined using Combined Disk. Subsequently, the Multiplex PCR approach with specific primers was employed to determine the presence of 6 subgroups of blaAmp-C genes. Results: Patterns of resistance to 14 antibiotics for isolates was identified. In combined disk test, a ≤5mm increase in zone diameter of FOX tested in combination with Clavulanic acid and a ≤3mm increase in combination with Boronic acid and Clavulanic acid, was considered positive for Amp-C production. With combined disk method 118 strains (59%) were producing Amp-C enzyme. 26 strains with Boronic acid and 92 strains with both inhibitors were considered Amp-C phenotype, among 118 strains. Prevalence of Amp-C enzyme was 2.5% that 4 strains were CITM positive and 1 strain was both DHA and CITM positive. Conclusion: The results of phenotypic tests in this study indicate that Amp-C β-lactamase enzyme has high frequency (59%); however, low frequency of this enzyme is observed when plasmid-mediated Amp-C β lactamase was used in PCR.

Item Type: Article
Keywords: words:Combined disk, Amp-C, Boronic acid, Clavulanic acid, E.coli, Multiplex PCR
Subjects: molecular Genetics
Divisions: Research Vice-Chancellor Department > Molecular Medicine Research Center
Depositing User: مرکز تحقیقات پزشکی مولکولی
URI: http://eprints.hums.ac.ir/id/eprint/1043

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